Hotspot

petN-psbM

LSC · 28764-29492 · 729 bp

Back to Epipactis

Pi 0.0157

MarkerSeek score 54.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 32
pi: 0.0157
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCCTATTTTGACTCTGCGCC	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	984-1000 bp	1.0	83.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	883-899 bp	1.0	82.6
petN-psbM_p3	TTGACTCTGCGCCATTGATT	CGTTTATTGCTACTGCGCTG	45.0	50.0	58.2	57.9	977-993 bp	1.0	82.0
petN-psbM_p4	TTTGACTCTGCGCCATTGAT	CGTTTATTGCTACTGCGCTG	45.0	50.0	58.2	57.9	978-994 bp	1.0	82.0
petN-psbM_p5	TCCTATTTTGACTCTGCGCC	AGCGGGAAAATGAAATGGGA	50.0	45.0	58.0	57.8	1276-1292 bp	0.875	76.3