Hotspot

trnS-GCU-trnG-GCC

LSC · 8155-8919 · 765 bp

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Pi 0.0141

MarkerSeek score 62.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 29
pi: 0.0141
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	TTAGTCCCCTCAGCCATCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	781-843 bp	1.0	56.9
trnS-GCU-trnG-GCC_p2	TTTAGTCCCCTCAGCCATCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	782-844 bp	1.0	56.4
trnS-GCU-trnG-GCC_p3	TTAGTCCCCTCAGCCATCTC	AGAACGAATCACACTTTTACCAC	55.0	39.1	58.2	57.3	784-846 bp	1.0	55.8
trnS-GCU-trnG-GCC_p4	TTAGTCCCCTCAGCCATCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.2	57.3	783-845 bp	1.0	55.8
trnS-GCU-trnG-GCC_p5	CGGAGAGAGAGGGATTCGAA	ACGAATCACACTTTTACCACT	55.0	38.1	58.3	55.5	846-908 bp	1.0	55.6