Hotspot

matK-trnK-UUU

LSC · 3232-4111 · 880 bp

Back to Epipactis

Pi 0.00588

MarkerSeek score 57.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 16
pi: 0.00588
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTTGTAATTCTGCCATTTACA	TCCGGGTTGCTAACTCAATG	34.8	50.0	56.2	57.9	906-949 bp	1.0	45.8
matK-trnK-UUU_p2	TCCTTGTAATTCTGCCATTTACA	CGGGTTGCTAACTCAATGGT	34.8	50.0	56.2	58.2	904-947 bp	1.0	45.4
matK-trnK-UUU_p3	TCCTTGTAATTCTGCCATTTACA	GATCCGGGTTGCTAACTCAA	34.8	50.0	56.2	57.6	908-951 bp	1.0	45.4
matK-trnK-UUU_p4	TCCTTGTAATTCTGCCATTTACA	TTGATCCGGGTTGCTAACTC	34.8	50.0	56.2	57.6	910-953 bp	1.0	45.4
matK-trnK-UUU_p5	CCTTGTAATTCTGCCATTTACA	TCCGGGTTGCTAACTCAATG	36.4	50.0	54.7	57.9	905-948 bp	1.0	45.4