Hotspot

psbA-trnK-UUU

LSC · 1165-1410 · 246 bp

Back to Epipactis

Pi 0.0247

MarkerSeek score 60.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 14
pi: 0.0247
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	303-334 bp	1.0	82.5
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.0	58.8	293-324 bp	1.0	80.8
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.0	58.8	294-325 bp	1.0	80.8
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.0	58.9	298-329 bp	1.0	80.3
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.3	58.6	300-331 bp	1.0	80.2