Hotspot

ndhF-rpl32

SSC · 116631-117348 · 718 bp

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Pi 0.0335

MarkerSeek score 82.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 718
variable_sites: 98
pi: 0.0335
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	GCTGCCCAATATGCCTTTTT	45.0	45.0	58.0	57.3	850-915 bp	0.933333	83.4
ndhF-rpl32_p2	TGCTGTCGGAAAAAGGAGAA	GCTGCCCAATATGCCTTTTT	45.0	45.0	57.4	57.3	847-912 bp	0.933333	78.7
ndhF-rpl32_p3	TTGCTGTCGGAAAAAGGAGA	GCTGCCCAATATGCCTTTTT	45.0	45.0	57.4	57.3	848-913 bp	0.933333	78.7
ndhF-rpl32_p4	GTTGCTGTCGGAAAAAGGAG	GCTGCCCAATATGCCTTTTT	50.0	45.0	57.3	57.3	849-914 bp	0.933333	78.3
ndhF-rpl32_p5	CGGAAAAAGGAGAAGTCCCA	GCTGCCCAATATGCCTTTTT	50.0	45.0	57.4	57.3	841-898 bp	0.866667	75.4