Hotspot

petN-psbM

LSC · 30172-31076 · 905 bp

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Pi 0.0125

MarkerSeek score 69.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 52
pi: 0.0125
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	ACGTAAAAACAGTCAGTCAACG	45.0	40.9	58.0	57.5	767-1047 bp	1.0	68.1
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	CGTAAAAACAGTCAGTCAACGT	45.0	40.9	58.0	57.5	766-1046 bp	1.0	68.1
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AAAACAGTCAGTCAACGTGA	45.0	40.0	58.0	55.5	762-1042 bp	1.0	68.0
petN-psbM_p4	CTTTCACTGGTGGTATGGGG	ACGTAAAAACAGTCAGTCAACG	55.0	40.9	57.9	57.5	727-1007 bp	1.0	67.3
petN-psbM_p5	CTTTCACTGGTGGTATGGGG	CGTAAAAACAGTCAGTCAACGT	55.0	40.9	57.9	57.5	726-1006 bp	1.0	67.3