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trnC-GCA-petN

LSC · 29169-30081 · 913 bp

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Pi 0.0126

MarkerSeek score 65.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 913
variable_sites: 49
pi: 0.0126
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TATCATTCTGGCGACATGGC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	1022-1034 bp	1.0	83.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	971-983 bp	0.933333	80.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CCATACCACCAGTGAAAGGG	50.0	55.0	58.1	57.9	1009-1021 bp	0.933333	79.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CCCATACCACCAGTGAAAGG	50.0	55.0	58.1	57.9	1010-1022 bp	0.933333	79.7
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CCCCATACCACCAGTGAAAG	50.0	55.0	58.1	57.9	1011-1023 bp	0.933333	79.7