Hotspot

atpF-atpH

LSC · 13927-14432 · 506 bp

Back to Epilobium

Pi 0.0127

MarkerSeek score 70.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 33
pi: 0.0127
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	636-706 bp	1.0	81.2
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTAGCCTTAGCA	50.0	47.6	57.9	58.0	565-635 bp	1.0	79.3
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGATTGGTTGTAGCCTTAGC	50.0	47.6	57.9	58.0	566-636 bp	1.0	79.3
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	631-701 bp	1.0	78.8
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCCTTAGCACTT	50.0	45.0	57.9	56.8	561-631 bp	1.0	77.4