Hotspot

trnR-UCU-atpA

LSC · 10831-11053 · 223 bp

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Pi 0.014

MarkerSeek score 71.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 223
variable_sites: 13
pi: 0.014
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TCGGAATCAAAAAGGCGTCC	CGCTATTCAGGAGCAGAAGG	50.0	55.0	58.8	58.1	352-385 bp	1.0	79.3
trnR-UCU-atpA_p2	TCGGAATCAAAAAGGCGTCC	CACAAGCCCTTTTGAACGAC	50.0	50.0	58.8	57.9	371-404 bp	1.0	79.2
trnR-UCU-atpA_p3	TCGGAATCAAAAAGGCGTCC	GAGCAGAAGGAACGCTTTCT	50.0	50.0	58.8	58.2	342-375 bp	1.0	78.9
trnR-UCU-atpA_p4	TCGGAATCAAAAAGGCGTCC	ATTCACCGAGGAAGCACAAG	50.0	50.0	58.8	58.2	385-418 bp	1.0	78.5
trnR-UCU-atpA_p5	TCGGAATCAAAAAGGCGTCC	ACCGCAGTTCGAAGAAATCA	50.0	45.0	58.8	57.8	421-450 bp	0.933333	75.3