Hotspot

rps16-trnQ-UUG

LSC · 6154-7586 · 1433 bp

Back to Epilobium

Pi 0.0144

MarkerSeek score 69.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1433
variable_sites: 90
pi: 0.0144
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGCGTTGCTTTTTACCACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1020-1529 bp	1.0	88.4
rps16-trnQ-UUG_p2	ATCATGTCTGTCAAGTCGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1038-1547 bp	1.0	85.7
rps16-trnQ-UUG_p3	CGCGTTGCTTTTTACCACAT	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.0	57.3	1024-1533 bp	1.0	83.6
rps16-trnQ-UUG_p4	CGCGTTGCTTTTTACCACAT	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.0	58.7	1050-1559 bp	1.0	83.4
rps16-trnQ-UUG_p5	CGCGTTGCTTTTTACCACAT	TAGAAATCGAAATGGGGCGT	45.0	45.0	58.0	57.3	1080-1589 bp	1.0	83.1