Hotspot

trnK-UUU-rps16

LSC · 4256-5013 · 758 bp

Back to Epilobium

Pi 0.0185

MarkerSeek score 82.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 60
pi: 0.0185
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.3	58.0	854-908 bp	1.0	87.3
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	AGAAAGCGGGGGTTTTTACC	50.0	50.0	58.3	58.4	813-867 bp	1.0	84.6
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	AAAAAGGAGCTCAACCCACA	50.0	45.0	58.3	57.5	855-909 bp	1.0	83.8
trnK-UUU-rps16_p4	GTGTAAAAGATGCTAGCCGC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.3	58.0	870-924 bp	1.0	83.6
trnK-UUU-rps16_p5	CGCACTTAAAAGCCGAGTAC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.2	58.0	853-907 bp	1.0	83.3