Hotspot

ycf1

SSC · 118852-124284 · 5433 bp

Back to Epidendrum

Pi 0.0259

MarkerSeek score 60.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5433
variable_sites: 373
pi: 0.0259
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAAGGTCGTTTTCGGGGA	GGGCCTCTTTCAAGCAAATG	45.0	50.0	57.9	57.6	647-662 bp	1.0	85.1
ycf1_p2	TTTTCGGGGAGAACCAAAGG	GGGCCTCTTTCAAGCAAATG	50.0	50.0	58.0	57.6	637-652 bp	0.833333	78.5
ycf1_p3	TTTTCGGGGAGAACCAAAGG	CGCGCTCAAAGACGTAAAAC	50.0	50.0	58.0	58.4	666-681 bp	0.833333	77.9
ycf1_p4	TTTTCGGGGAGAACCAAAGG	TCCGTAGAACTTCACCGTCA	50.0	50.0	58.0	58.4	1161-1167 bp	0.666667	68.8
ycf1_p5	TTTTCGGGGAGAACCAAAGG	CCGTAGAACTTCACCGTCAA	50.0	50.0	58.0	57.6	1160-1166 bp	0.666667	68.5