Hotspot

ndhE

SSC · 117566-117748 · 183 bp

Back to Epidendrum

Pi 0.0423

MarkerSeek score 75.10

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 183
variable_sites: 21
pi: 0.0423
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	AGCTCGAACACATTGGGTAC	ATGGTGTTCTTCGGCTGATC	50.0	50.0	57.9	58.0	1280-1321 bp	0.666667	72.8
ndhE_p2	AGCTCGAACACATTGGGTAC	ACCCATCAATGCATGGTGTT	50.0	45.0	57.9	58.1	1292-1333 bp	0.666667	72.5
ndhE_p3	AGCTCGAACACATTGGGTAC	TGACTGTACCGGCTACAAGA	50.0	50.0	57.9	58.1	1346-1387 bp	0.666667	72.5
ndhE_p4	ACATCCGTCCCAAGGTATCA	ATGGTGTTCTTCGGCTGATC	50.0	50.0	58.1	58.0	1322-1363 bp	0.666667	72.4
ndhE_p5	ACATCCGTCCCAAGGTATCA	ACCCATCAATGCATGGTGTT	50.0	45.0	58.1	58.1	1334-1375 bp	0.666667	72.2