Hotspot

trnN-GUU-ndhF

IRb · 110586-111511 · 926 bp

Back to Epidendrum

Pi 0.058

MarkerSeek score 65.70

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 926
variable_sites: 58
pi: 0.058
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.33
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGTCAGTTAACAGCCAACCG	ACTACTGTTGGAATCATGGT	50.0	40.0	58.1	53.9	1005-1011 bp	0.333333	27.8
trnN-GUU-ndhF_p2	ACGACCAGTCAGTTAACAGC	ACTACTGTTGGAATCATGGT	50.0	40.0	57.8	53.9	1011-1017 bp	0.333333	27.5
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	ACTACTGTTGGAATCATGGT	50.0	40.0	58.2	53.9	1024-1030 bp	0.333333	27.4
trnN-GUU-ndhF_p4	GGATTCGAACCTACGACCAG	ACTACTGTTGGAATCATGGT	55.0	40.0	57.8	53.9	1023-1029 bp	0.333333	27.0
trnN-GUU-ndhF_p5	AGTCAGTTAACAGCCAACCG	ACTACTGTTGGAATCATGGTT	50.0	38.1	58.1	54.8	1005-1011 bp	0.333333	26.9