Hotspot

petD-rpoA

LSC · 79253-79433 · 181 bp

Back to Epidendrum

Pi 0.0431

MarkerSeek score 76.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 18
pi: 0.0431
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AGGTATTGGAGCAACTTTACCT	CACTTTGCAATCAATTTACCT	40.9	33.3	57.1	53.0	282-292 bp	1.0	46.7
petD-rpoA_p2	TGGTTAGGTATTGGAGCAACT	CACTTTGCAATCAATTTACCT	42.9	33.3	56.6	53.0	287-297 bp	1.0	46.6
petD-rpoA_p3	TGGTTAGGTATTGGAGCAACTT	CACTTTGCAATCAATTTACCT	40.9	33.3	57.3	53.0	287-297 bp	1.0	46.6
petD-rpoA_p4	TTGGTTAGGTATTGGAGCAACT	CACTTTGCAATCAATTTACCT	40.9	33.3	57.3	53.0	288-298 bp	1.0	46.6
petD-rpoA_p5	TCTTTGGTTAGGTATTGGAGCA	CACTTTGCAATCAATTTACCT	40.9	33.3	57.1	53.0	291-301 bp	1.0	46.5