Hotspot

psbT-psbN

LSC · 75622-75684 · 63 bp

Back to Epidendrum

Pi 0.0857

MarkerSeek score 74.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 10
pi: 0.0857
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGGGCTACTTTGAAATCCGA	CACACGCTCATATTCCGGAA	50.0	50.0	58.0	58.1	1138-1151 bp	1.0	83.3
psbT-psbN_p2	GGGATGGAATAGCTGTTGGG	CACACGCTCATATTCCGGAA	55.0	50.0	58.0	58.1	1440-1453 bp	1.0	82.9
psbT-psbN_p3	GGGGATGGAATAGCTGTTGG	CACACGCTCATATTCCGGAA	55.0	50.0	58.0	58.1	1441-1454 bp	1.0	82.9
psbT-psbN_p4	GGGATGGAATAGCTGTTGGG	CCCTAGTCGCCATCTCCATA	55.0	55.0	58.0	58.1	907-909 bp	1.0	82.5
psbT-psbN_p5	GGGGATGGAATAGCTGTTGG	CCCTAGTCGCCATCTCCATA	55.0	55.0	58.0	58.1	908-910 bp	1.0	82.5