Hotspot

psaJ-rpl33

LSC · 68162-68687 · 526 bp

Back to Epidendrum

Pi 0.0325

MarkerSeek score 74.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 47
pi: 0.0325
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AGGGGCGGTAAAATGGAATC	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.9	58.0	996-1056 bp	0.5	63.8
psaJ-rpl33_p2	AGGGGCGGTAAAATGGAATC	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.9	58.0	995-1055 bp	0.5	63.8
psaJ-rpl33_p3	GGGGCGGTAAAATGGAATCT	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.9	58.0	995-1055 bp	0.5	63.8
psaJ-rpl33_p4	GGGGCGGTAAAATGGAATCT	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.9	58.0	994-1054 bp	0.5	63.8
psaJ-rpl33_p5	GGGGCGGTAAAATGGAATCT	GATCGACTCCACTCTCTCCA	50.0	55.0	57.9	58.0	1558-1624 bp	0.5	62.5