Hotspot

psbK-psbI

LSC · 7524-7949 · 426 bp

Back to Epidendrum

Pi 0.0418

MarkerSeek score 77.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 41
pi: 0.0418
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTGTTTGGCAAGCTGCTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	508-589 bp	1.0	88.9
psbK-psbI_p2	CTTTGTTTGGCAAGCTGCTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	509-590 bp	1.0	88.9
psbK-psbI_p3	CTTTGTTTGGCAAGCTGCTT	TTACGCCCCGGATCATTAGA	45.0	50.0	58.1	58.3	510-591 bp	1.0	87.0
psbK-psbI_p4	CTTTGTTTGGCAAGCTGCTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.1	57.6	526-607 bp	1.0	86.1
psbK-psbI_p5	TTTGTTTGGCAAGCTGCTTT	ACGCCCCGGATCATTAGATA	40.0	50.0	57.6	58.1	507-588 bp	1.0	86.1