Hotspot

rpl2

Genome · 58726-60001 · 1276 bp

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Pi 0.00598

MarkerSeek score 48.80

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 1276
variable_sites: 48
pi: 0.00598
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAGTGTGTAGCCGCAAAAAC	ACCAGATACCTACTACGGGC	50.0	55.0	58.0	58.0	1552-1639 bp	1.0	87.0
rpl2_p2	GAGTGTGTAGCCGCAAAAAC	CAGATACCTACTACGGGCCA	50.0	55.0	58.0	58.0	1550-1637 bp	1.0	87.0
rpl2_p3	GAGTGTGTAGCCGCAAAAAC	TCCATTCAAGTTGAACGCCA	50.0	45.0	58.0	58.0	1596-1683 bp	1.0	86.8
rpl2_p4	GAGTGTGTAGCCGCAAAAAC	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	1972-2059 bp	1.0	86.3
rpl2_p5	AAGGAACGTCCCTTAGCTCT	ACCAGATACCTACTACGGGC	50.0	55.0	58.1	58.0	2166-2253 bp	1.0	86.1