Hotspot

psaC

Genome · 81038-81283 · 246 bp

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Pi 0.00377

MarkerSeek score 23.30

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: Genome
length_bp: 246
variable_sites: 4
pi: 0.00377
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TAGATCGGGCTACTCTGGTG	TCGTTGGTTGGGTGCAATTA	55.0	45.0	58.0	58.0	1644-1686 bp	1.0	88.1
psaC_p2	CAATGCAAGAGACGTGCTTG	TCGTTGGTTGGGTGCAATTA	50.0	45.0	58.0	58.0	1709-1751 bp	1.0	87.8
psaC_p3	TAGATCGGGCTACTCTGGTG	CGTTGGTTGGGTGCAATTAC	55.0	50.0	58.0	57.9	1643-1685 bp	1.0	87.8
psaC_p4	TAGATCGGGCTACTCTGGTG	TATTCGTTGGTTGGGTGCAA	55.0	45.0	58.0	58.0	1647-1689 bp	0.971429	86.9
psaC_p5	CAATGCAAGAGACGTGCTTG	TATTCGTTGGTTGGGTGCAA	50.0	45.0	58.0	58.0	1712-1754 bp	0.971429	86.5