Hotspot

rps15-psaC

Genome · 80732-81037 · 306 bp

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Pi 0.0257

MarkerSeek score 68.50

Primer available yes

Label rps15-psaC

Feature Metadata

label_name: rps15-psaC
feature_type: igs
region: Genome
length_bp: 306
variable_sites: 43
pi: 0.0257
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-psaC_p1	CAAAGGAGTCTGCGGAAACT	AATTTTGACTGAATGAGCCA	50.0	35.0	58.1	53.3	400-454 bp	1.0	53.8
rps15-psaC_p2	TGATCAAACGGAAGCGACTT	AATTTTGACTGAATGAGCCA	45.0	35.0	57.8	53.3	378-432 bp	1.0	53.5
rps15-psaC_p3	TTGATCAAACGGAAGCGACT	AATTTTGACTGAATGAGCCA	45.0	35.0	57.8	53.3	379-433 bp	1.0	53.5
rps15-psaC_p4	CAAAGGAGTCTGCGGAAACT	GTACAACCAATGCATGTATCA	50.0	38.1	58.1	54.3	423-477 bp	1.0	53.2
rps15-psaC_p5	CAAAGGAGTCTGCGGAAACT	AAATTTTGACTGAATGAGCCA	50.0	33.3	58.1	54.2	401-455 bp	1.0	53.1