Hotspot

rps11-rpl36

Genome · 54630-54712 · 83 bp

Back to Ephedra

Pi 0.0351

MarkerSeek score 58.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: Genome
length_bp: 83
variable_sites: 11
pi: 0.0351
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCCACATAGATTTTGGTTTCA	TCGTAGAGGAAAGCGAATTT	33.3	40.0	53.0	54.8	169-186 bp	1.0	47.8
rps11-rpl36_p2	TCCACATAGATTTTGGTTTCA	TTCGTAGAGGAAAGCGAATT	33.3	40.0	53.0	54.8	170-187 bp	1.0	47.8
rps11-rpl36_p3	TTCCACATAGATTTTGGTTTCA	TCGTAGAGGAAAGCGAATTT	31.8	40.0	53.9	54.8	170-187 bp	1.0	47.8
rps11-rpl36_p4	TCCACATAGATTTTGGTTTCA	AATTCGTAGAGGAAAGCGAA	33.3	40.0	53.0	54.8	172-189 bp	1.0	47.7
rps11-rpl36_p5	TTCCACATAGATTTTGGTTTCA	TTCGTAGAGGAAAGCGAATT	31.8	40.0	53.9	54.8	171-188 bp	1.0	47.7