Hotspot

psaJ-rpl33

Genome · 44910-45033 · 124 bp

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Pi 0.0247

MarkerSeek score 65.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 124
variable_sites: 12
pi: 0.0247
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTCGACAGCACCCGTTTTA	TGCGCCTATGTTTTCTGACA	45.0	45.0	58.0	57.8	242-258 bp	1.0	86.7
psaJ-rpl33_p2	TTTCGACAGCACCCGTTTTA	AAAAATTACCACTCGTGCGC	45.0	45.0	58.0	57.7	257-273 bp	1.0	85.3
psaJ-rpl33_p3	ATATCTTTCGACAGCACCCG	TGCGCCTATGTTTTCTGACA	50.0	45.0	57.8	57.8	247-263 bp	1.0	85.2
psaJ-rpl33_p4	ATATCTTTCGACAGCACCCG	AAAAATTACCACTCGTGCGC	50.0	45.0	57.8	57.7	262-278 bp	1.0	83.8
psaJ-rpl33_p5	AGCACCCGTTTTAGCAACTT	TGCGCCTATGTTTTCTGACA	45.0	45.0	58.3	57.8	235-251 bp	0.942857	82.1