Hotspot

psbJ-psbL

Genome · 43036-43160 · 125 bp

Back to Ephedra

Pi 0.0198

MarkerSeek score 57.90

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: Genome
length_bp: 125
variable_sites: 11
pi: 0.0198
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TTCTCCCGGTAGTATCAGCC	CAGCCTATACTGGGGATTGT	55.0	50.0	58.3	56.4	212-226 bp	1.0	72.6
psbJ-psbL_p2	CTCCCGGTAGTATCAGCCAT	CAGCCTATACTGGGGATTGT	55.0	50.0	58.4	56.4	210-224 bp	1.0	72.1
psbJ-psbL_p3	TTCTCCCGGTAGTATCAGCC	TCCGAACGAACAAAGTGTAGA	55.0	42.9	58.3	57.3	245-259 bp	1.0	71.4
psbJ-psbL_p4	CTCCCGGTAGTATCAGCCAT	TCCGAACGAACAAAGTGTAGA	55.0	42.9	58.4	57.3	243-257 bp	1.0	70.9
psbJ-psbL_p5	TTCTCCCGGTAGTATCAGCC	TGACACAATCAAATCCGAACG	55.0	42.9	58.3	57.2	258-272 bp	1.0	70.4