Hotspot

trnfM-CAU-atpE

Genome · 36015-36078 · 64 bp

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Pi 0.0569
MarkerSeek score 73.80
Primer available yes
Label trnfM-CAU-atpE

Feature Metadata

label_name
trnfM-CAU-atpE
feature_type
igs
region
Genome
length_bp
64
variable_sites
16
pi
0.0569
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-atpE_p1 AAGGCGAGAGTCATTGGTTC ACGCAAATCGAGTTCTAGGC 50.0 50.0 57.9 58.4 187-210 bp 0.942857 85.8
trnfM-CAU-atpE_p2 AAGGCGAGAGTCATTGGTTC CGCAAATCGAGTTCTAGGCA 50.0 50.0 57.9 58.4 186-209 bp 0.942857 85.8
trnfM-CAU-atpE_p3 CGCTTTCATAAGGCGAGAGT ACGCAAATCGAGTTCTAGGC 50.0 50.0 58.1 58.4 196-219 bp 0.942857 85.7
trnfM-CAU-atpE_p4 CGCTTTCATAAGGCGAGAGT CGCAAATCGAGTTCTAGGCA 50.0 50.0 58.1 58.4 195-218 bp 0.942857 85.7
trnfM-CAU-atpE_p5 GGCGAGAGTCATTGGTTCAA ACGCAAATCGAGTTCTAGGC 50.0 50.0 58.2 58.4 185-208 bp 0.942857 85.1