Hotspot

trnR-UCU-trnS-GCU

Genome · 32665-33429 · 765 bp

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Pi 0.0127

MarkerSeek score 60.20

Primer available yes

Label trnR-UCU-trnS-GCU

Feature Metadata

label_name: trnR-UCU-trnS-GCU
feature_type: igs
region: Genome
length_bp: 765
variable_sites: 40
pi: 0.0127
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnS-GCU_p1	AGAAGACCTCTGTCCTATCCA	CTTTGGTCCTCTCAGCCATC	47.6	55.0	56.9	58.0	793-856 bp	1.0	71.2
trnR-UCU-trnS-GCU_p2	AGGTTTAGAAGACCTCTGTCCT	CTTTGGTCCTCTCAGCCATC	45.5	55.0	57.9	58.0	799-862 bp	1.0	70.5
trnR-UCU-trnS-GCU_p3	AGAAGACCTCTGTCCTATCCA	CGGAGAGAGAGGGATTCGAA	47.6	55.0	56.9	58.3	857-920 bp	1.0	68.8
trnR-UCU-trnS-GCU_p4	AGAAGACCTCTGTCCTATCCA	GAACCCTCGATACGGGATTG	47.6	55.0	56.9	57.8	840-903 bp	0.942857	67.7
trnR-UCU-trnS-GCU_p5	AGGTTTAGAAGACCTCTGTCCT	GAACCCTCGATACGGGATTG	45.5	55.0	57.9	57.8	846-909 bp	0.942857	67.0