Hotspot

rpoC2-rps2

Genome · 27576-27707 · 132 bp

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Pi 0.0458

MarkerSeek score 73.20

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: Genome
length_bp: 132
variable_sites: 24
pi: 0.0458
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	GCCGGTACTGGAAAATGGAA	TGATGTCCGAGATGAACTGC	50.0	50.0	58.2	57.7	197-280 bp	0.971429	86.7
rpoC2-rps2_p2	GCCGGTACTGGAAAATGGAA	GATGTCCGAGATGAACTGCT	50.0	50.0	58.2	57.4	196-279 bp	0.971429	84.4
rpoC2-rps2_p3	AGTAGTAAAATACCCGCCGG	TGATGTCCGAGATGAACTGC	50.0	50.0	57.1	57.7	194-295 bp	1.0	82.3
rpoC2-rps2_p4	GCCGGTACTGGAAAATGGAA	TGTCCGAGATGAACTGCTAC	50.0	50.0	58.2	57.1	194-277 bp	0.971429	81.7
rpoC2-rps2_p5	GCCGGTACTGGAAAATGGAA	GTCCGAGATGAACTGCTACT	50.0	50.0	58.2	56.8	193-276 bp	0.971429	79.3