Hotspot

rpl16

LSC · 78089-79629 · 1541 bp

Back to Enteropogon

Pi 0.0204

MarkerSeek score 59.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1541
variable_sites: 30
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	GGGTAGACTTCCCCTCCAAA	50.0	55.0	58.1	58.3	2169-2221 bp	1.0	81.1
rpl16_p2	TCTCAATTCCTCGGCAATCG	GGAATCAGACCCAAGTTCCG	50.0	55.0	58.1	58.3	2605 bp	0.5	57.4
rpl16_p3	GGGGTACTGCGTCTTTGATT	GGAATCAGACCCAAGTTCCG	50.0	55.0	57.9	58.3	2429 bp	0.5	57.0
rpl16_p4	TCAGCAATAGTGTCCTTGCC	GGAATCAGACCCAAGTTCCG	50.0	55.0	57.9	58.3	2815 bp	0.5	57.0
rpl16_p5	GCTCCACTGTTATCCGCTAC	GGAATCAGACCCAAGTTCCG	55.0	55.0	58.1	58.3	2328 bp	0.5	56.7