Hotspot

ndhF-rpl32

SSC · 104200-105073 · 874 bp

Back to Enteropogon

Pi 0.0289

MarkerSeek score 70.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 874
variable_sites: 24
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	916-957 bp	1.0	46.8
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	917-958 bp	1.0	46.8
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	918-959 bp	1.0	46.8
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	919-960 bp	1.0	46.7
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	920-961 bp	1.0	46.7