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petG-trnW-CCA

LSC · 64243-64366 · 124 bp

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Pi 0.0492

MarkerSeek score 71.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 6
pi: 0.0492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCGTATTTGCAATACAGGCG	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.3	57.9	203-205 bp	1.0	80.4
petG-trnW-CCA_p2	TCGTGACTGCGTATTTGCAA	CAAAACCCGATGTCGTAGGT	45.0	50.0	58.5	57.9	211-213 bp	1.0	78.3
petG-trnW-CCA_p3	TTCGTGACTGCGTATTTGCA	CAAAACCCGATGTCGTAGGT	45.0	50.0	58.5	57.9	212-214 bp	1.0	78.3
petG-trnW-CCA_p4	GCGTATTTGCAATACAGGCG	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.3	57.6	198-200 bp	1.0	78.2
petG-trnW-CCA_p5	CGTGACTGCGTATTTGCAAT	CAAAACCCGATGTCGTAGGT	45.0	50.0	57.5	57.9	210-212 bp	1.0	78.1