Hotspot

trnF-GAA-ndhJ

LSC · 48476-48976 · 501 bp

Back to Enteropogon

Pi 0.0384

MarkerSeek score 75.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 19
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	611-614 bp	1.0	81.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	601-604 bp	1.0	81.3
trnF-GAA-ndhJ_p3	ATGCACAAGAAAGGGGTCAG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	665 bp	0.5	58.3
trnF-GAA-ndhJ_p4	GATGCACAAGAAAGGGGTCA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	666 bp	0.5	58.2
trnF-GAA-ndhJ_p5	TGATGCACAAGAAAGGGGTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	667 bp	0.5	58.2