Hotspot

trnR-UCU-rps14

LSC · 36745-37138 · 394 bp

Back to Enteropogon

Pi 0.0368

MarkerSeek score 71.10

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 14
pi: 0.0368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	ATGCATGTTTGTTACCGGGT	47.6	45.0	56.9	58.1	459-471 bp	1.0	67.7
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	TCCGGACACATACTTCGAGA	47.6	50.0	56.9	57.9	487-499 bp	1.0	67.2
trnR-UCU-rps14_p3	AGGTTTAGAAGACCTCTGTCCT	ATGCATGTTTGTTACCGGGT	45.5	45.0	57.9	58.1	465-477 bp	1.0	67.0
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	448-460 bp	1.0	67.0
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	TCCGGACACATACTTCGAGA	45.5	50.0	57.9	57.9	493-505 bp	1.0	66.5