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trnC-GCA-rpoB

LSC · 18888-19944 · 1057 bp

Back to Enteropogon

Pi 0.0339

MarkerSeek score 72.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 35
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1117-1141 bp	1.0	81.0
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1116-1140 bp	1.0	81.0
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1100-1124 bp	1.0	80.4
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1101-1125 bp	1.0	80.2
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCCGGGTATTGTGGACATTC	50.0	50.0	58.1	57.3	1118-1142 bp	1.0	78.0