Hotspot

psbM-petN

LSC · 17246-17880 · 635 bp

Back to Enteropogon

Pi 0.0355

MarkerSeek score 76.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 18
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	569-684 bp	1.0	75.0
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	568-683 bp	1.0	75.0
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	627-742 bp	1.0	74.5
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	567-682 bp	1.0	74.5
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	564-679 bp	1.0	74.2