Hotspot

trnD-GUC-psbM

LSC · 16078-17092 · 1015 bp

Back to Enteropogon

Pi 0.0353

MarkerSeek score 73.70

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 35
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.6	57.9	1179-1186 bp	1.0	55.0
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	GGCAGTAGGAACTAGAATGAACA	55.0	43.5	58.7	57.9	1212-1219 bp	1.0	54.0
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	58.6	57.7	1180-1187 bp	1.0	52.9
trnD-GUC-psbM_p4	TAGGTATGCCATACACCCCG	AGGCAGTAGGAACTAGAATGAAC	55.0	43.5	58.7	57.7	1213-1220 bp	1.0	51.9
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	58.6	55.5	1180-1187 bp	1.0	51.6