Hotspot

trnY-GUA-trnD-GUC

LSC · 15648-16003 · 356 bp

Back to Enteropogon

Pi 0.0554
MarkerSeek score 78.00
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
356
variable_sites
19
pi
0.0554
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC GCGGTGCTCTAACCAATTGA 50.0 50.0 58.0 58.6 477-481 bp 1.0 80.6
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG GCGGTGCTCTAACCAATTGA 55.0 50.0 58.4 58.6 444-448 bp 1.0 78.5
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT GCGGTGCTCTAACCAATTGA 50.0 50.0 57.6 58.6 443-447 bp 1.0 78.3
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGCGGTGCTCTAACCAATT 50.0 52.6 58.0 58.1 478-482 bp 1.0 75.9
trnY-GUA-trnD-GUC_p5 TGGGGACGGACTGTAAATTC GCGGTGCTCTAACCAATTGA 50.0 50.0 57.2 58.6 445-449 bp 1.0 75.1