Hotspot

rps16-trnQ-UUG

LSC · 5595-6855 · 1261 bp

Back to Enteropogon

Pi 0.0247

MarkerSeek score 65.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1261
variable_sites: 31
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1334-1347 bp	1.0	80.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CGCATAAAAGAAATGGGGCG	45.0	50.0	58.1	58.2	1395-1408 bp	1.0	79.6
rps16-trnQ-UUG_p3	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1345-1358 bp	1.0	78.5
rps16-trnQ-UUG_p4	TCATCTCCTTCAAGTCGCAC	CGCATAAAAGAAATGGGGCG	50.0	50.0	57.6	58.2	1406-1419 bp	1.0	77.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1338-1351 bp	1.0	75.6