Hotspot

trnN-trnR

IRa · 134151-134762 · 612 bp

Back to Entandrophragma

Pi 0.0079

MarkerSeek score 64.30

Primer available yes

Label trnN-trnR

Feature Metadata

label_name: trnN-trnR
feature_type: igs
region: IRa
length_bp: 612
variable_sites: 9
pi: 0.0079
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-trnR_p1	TGGGTTGAGAACGGGAATTG	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.1	58.1	845-847 bp	1.0	79.0
trnN-trnR_p2	GCTGTTAACTGACTGGTCGT	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	768-770 bp	1.0	78.7
trnN-trnR_p3	TGGTCGTAGGTTCGAATCCT	TAGGGTACTTCCGGGAACAG	50.0	55.0	58.2	58.1	755-757 bp	1.0	78.7
trnN-trnR_p4	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	794-796 bp	1.0	78.5
trnN-trnR_p5	GGGTTGAGAACGGGAATTGA	TAGGGTACTTCCGGGAACAG	50.0	55.0	57.8	58.1	844-846 bp	1.0	78.2