Hotspot

ndhI-ndhA

SSC · 124162-124242 · 81 bp

Back to Entandrophragma

Pi 0.0247

MarkerSeek score 69.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 81
variable_sites: 4
pi: 0.0247
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	ACCAATGTACCTTGCAGCTC	TCTTCCCAACTCCTTTCCCT	50.0	50.0	58.2	57.9	174 bp	1.0	79.4
ndhI-ndhA_p2	GACCAATGTACCTTGCAGCT	TCTTCCCAACTCCTTTCCCT	50.0	50.0	58.2	57.9	175 bp	1.0	79.4
ndhI-ndhA_p3	ACCTTGCAGCTCGTATTGTT	TCTTCCCAACTCCTTTCCCT	45.0	50.0	57.8	57.9	166 bp	1.0	79.4
ndhI-ndhA_p4	ACCAATGTACCTTGCAGCTC	CTTCCCAACTCCTTTCCCTG	50.0	55.0	58.2	57.8	173 bp	1.0	78.8
ndhI-ndhA_p5	GACCAATGTACCTTGCAGCT	CTTCCCAACTCCTTTCCCTG	50.0	55.0	58.2	57.8	174 bp	1.0	78.8