Hotspot

ndhD-psaC

SSC · 121651-121788 · 138 bp

Back to Entandrophragma

Pi 0.0195

MarkerSeek score 63.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 138
variable_sites: 5
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCGGCGATTGGAAAAACTAC	AAACAACTCGAAGCATGGGT	50.0	45.0	58.1	58.0	239-247 bp	1.0	83.2
ndhD-psaC_p2	GCGGCGATTGGAAAAACTAC	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	245-253 bp	1.0	83.0
ndhD-psaC_p3	GCGGCGATTGGAAAAACTAC	ACTGTGTTGGTTGTAAGCGA	50.0	45.0	58.1	57.7	314-322 bp	1.0	79.8
ndhD-psaC_p4	GCGGCGATTGGAAAAACTAC	CAGAGGACTGTGTTGGTTGT	50.0	50.0	58.1	57.7	320-328 bp	1.0	79.7
ndhD-psaC_p5	GCGGCGATTGGAAAAACTAC	GCCTGTCCAACGGATTTCTT	50.0	50.0	58.1	58.5	285-293 bp	1.0	79.0