Hotspot

ndhF-rpl32

SSC · 116394-117399 · 1006 bp

Back to Entandrophragma

Pi 0.0222

MarkerSeek score 64.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1006
variable_sites: 42
pi: 0.0222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTCCAAGACCCTAGGGATT	GGGAATGCAAGTGCGAGATA	55.0	50.0	58.1	58.0	1912-1943 bp	1.0	83.3
ndhF-rpl32_p2	GGTCCAAGACCCTAGGGATT	ATGCAAGTGCGAGATATGGG	55.0	50.0	58.1	58.1	1908-1939 bp	1.0	82.7
ndhF-rpl32_p3	GGGAATTGCGCAGATTTAGC	GGGAATGCAAGTGCGAGATA	50.0	50.0	57.9	58.0	2418-2449 bp	1.0	82.2
ndhF-rpl32_p4	GGGAATTGCGCAGATTTAGC	ATGCAAGTGCGAGATATGGG	50.0	50.0	57.9	58.1	2414-2445 bp	1.0	81.7
ndhF-rpl32_p5	GGTCCAAGACCCTAGGGATT	TCTGCCGAAATGGGAAAGAG	55.0	50.0	58.1	57.9	1973 bp	0.25	48.2