Hotspot

psbZ-trnG

LSC · 38423-39015 · 593 bp

Back to Entandrophragma

Pi 0.0156

MarkerSeek score 64.60

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 18
pi: 0.0156
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	720-743 bp	1.0	81.0
psbZ-trnG_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	730-753 bp	1.0	80.1
psbZ-trnG_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	731-754 bp	1.0	79.7
psbZ-trnG_p4	GGATGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	719-742 bp	1.0	77.8
psbZ-trnG_p5	CGTTGTATTTGCTTCTCCGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	737-760 bp	1.0	77.3