Hotspot

trnS-psbZ

LSC · 37886-38233 · 348 bp

Back to Entandrophragma

Pi 0.00575

MarkerSeek score 54.30

Primer available yes

Label trnS-psbZ

Feature Metadata

label_name: trnS-psbZ
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 4
pi: 0.00575
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbZ_p1	CAAGACCGGAGCTATCAACC	GTTACTCGACCAACCATCCG	55.0	55.0	58.1	58.4	481-492 bp	1.0	79.1
trnS-psbZ_p2	CTATCAACCACTCGGCCATC	GTTACTCGACCAACCATCCG	55.0	55.0	58.1	58.4	470-481 bp	1.0	78.6
trnS-psbZ_p3	CAAGACCGGAGCTATCAACC	CAACCATCCGGAGAAGCAAA	55.0	50.0	58.1	58.5	471-482 bp	1.0	78.3
trnS-psbZ_p4	TTCACCGAGCAAAAGGAGAG	GTTACTCGACCAACCATCCG	50.0	55.0	57.8	58.4	551-562 bp	1.0	77.9
trnS-psbZ_p5	CAAGACCGGAGCTATCAACC	AACCATCCGGAGAAGCAAAT	55.0	45.0	58.1	57.5	470-481 bp	1.0	77.9