Hotspot

psbC-trnS

LSC · 37556-37792 · 237 bp

Back to Entandrophragma

Pi 0.0281

MarkerSeek score 64.30

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 11
pi: 0.0281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	GCAGCAGGATTTGAAAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.6	58.1	383-385 bp	1.0	78.8
psbC-trnS_p2	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	387-389 bp	1.0	78.8
psbC-trnS_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	386-388 bp	1.0	78.3
psbC-trnS_p4	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	421-423 bp	1.0	78.2
psbC-trnS_p5	GCAGCAGGATTTGAAAAGGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.6	58.1	394-396 bp	1.0	78.2