Hotspot

petN-psbM

LSC · 30487-31583 · 1097 bp

Back to Entandrophragma

Pi 0.00628

MarkerSeek score 64.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 13
pi: 0.00628
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGGGCCGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1235-1247 bp	1.0	68.9
petN-psbM_p2	TTGGGCCGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1234-1246 bp	1.0	68.9
petN-psbM_p3	GGCCGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	1231-1243 bp	1.0	68.3
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1186-1198 bp	1.0	68.1
petN-psbM_p5	GGGCCGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.7	57.6	1232-1244 bp	1.0	65.3