Hotspot

trnR-atpA

LSC · 11211-11415 · 205 bp

Back to Entandrophragma

Pi 0.0138

MarkerSeek score 64.60

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 5
pi: 0.0138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	ATGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.5	58.1	338-363 bp	1.0	80.5
trnR-atpA_p2	GGAATGAAAGGCGTCCATTG	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	57.4	58.1	341-366 bp	1.0	79.9
trnR-atpA_p3	ATGAAAGGCGTCCATTGTCT	TTCACTGAGGAAGCAGAAGC	45.0	50.0	57.5	57.8	344-369 bp	1.0	79.7
trnR-atpA_p4	ATTCGGAATGAAAGGCGTCC	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.6	58.1	345-370 bp	1.0	79.5
trnR-atpA_p5	GGAATGAAAGGCGTCCATTG	TTCACTGAGGAAGCAGAAGC	50.0	50.0	57.4	57.8	347-372 bp	1.0	79.2