Hotspot

rps19

LSC · 87536-87814 · 279 bp

Back to Ensete

Pi 0.00896
MarkerSeek score 44.50
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
4
pi
0.00896
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 CGCGCTTTGAAAACAGACAT GTTATCCTGCGCTTGGAAGA 45.0 50.0 58.0 58.0 953-993 bp 1.0 87.0
rps19_p2 CGCGCTTTGAAAACAGACAT GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.0 58.0 1090-1130 bp 1.0 86.7
rps19_p3 CGCGCTTTGAAAACAGACAT TGGGTAGAGCCGGATCTAAG 45.0 55.0 58.0 58.0 1091-1131 bp 1.0 86.7
rps19_p4 CGCGCTTTGAAAACAGACAT GAGGGTAAATCGGCCACATT 45.0 50.0 58.0 57.9 1203-1243 bp 1.0 86.0
rps19_p5 TGCTGCTGCAGAATAAACCA GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.1 58.0 1205-1245 bp 1.0 86.0