Hotspot

psbA

LSC · 195-1256 · 1062 bp

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Pi 0.00738
MarkerSeek score 49.60
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
15
pi
0.00738
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AGCAATACCCCGTATCTTGC CATAGGGAAAGTCGTGTGCA 50.0 50.0 57.7 57.9 1541 bp 0.5 57.9
psbA_p2 GCAATACCCCGTATCTTGCT CATAGGGAAAGTCGTGTGCA 50.0 50.0 57.7 57.9 1540 bp 0.5 57.9
psbA_p3 GCAATACCCCGTATCTTGCT ACATAGGGAAAGTCGTGTGC 50.0 50.0 57.7 57.9 1541 bp 0.5 57.9
psbA_p4 AGCAATACCCCGTATCTTGC AATACGTGTGCTTGGGAGTC 50.0 50.0 57.7 57.9 1195 bp 0.5 57.8
psbA_p5 GCAATACCCCGTATCTTGCT AATACGTGTGCTTGGGAGTC 50.0 50.0 57.7 57.9 1194 bp 0.5 57.8