Hotspot

ndhD

SSC · 127909-129414 · 1506 bp

Pi 0.0134

MarkerSeek score 53.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 38
pi: 0.0134
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGGACCCCAAAGAAACTTG	CTGCTTCGTCTGGTGTCTAC	50.0	55.0	57.9	58.0	2409-2425 bp	1.0	82.2
ndhD_p2	GTGCGAGTTTTTGAGAACCG	CTGCTTCGTCTGGTGTCTAC	50.0	55.0	58.0	58.0	2135 bp	0.5	60.9
ndhD_p3	GTGCGAGTTTTTGAGAACCG	ACCGACAAAAACCCGTACTC	50.0	50.0	58.0	58.1	1760-1761 bp	0.5	60.1
ndhD_p4	GTGCGAGTTTTTGAGAACCG	CGTATGAAACAACTCGCAGC	50.0	50.0	58.0	57.9	1840-1841 bp	0.5	60.0
ndhD_p5	TTGGACCATATTCGCGGTTT	CTGCTTCGTCTGGTGTCTAC	45.0	55.0	57.9	58.0	2392 bp	0.25	48.9