Hotspot

ndhG-ndhI

SSC · 131456-131835 · 380 bp

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Pi 0.0352
MarkerSeek score 65.50
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
380
variable_sites
24
pi
0.0352
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 GACATTTACGGCTCCCACAT TTACGTCAGAGCGTTTTCGT 50.0 45.0 58.0 57.9 989-1012 bp 1.0 82.1
ndhG-ndhI_p2 ACTCCATCTCCAATGGTCCA TTACGTCAGAGCGTTTTCGT 50.0 45.0 58.0 57.9 1069-1092 bp 1.0 82.0
ndhG-ndhI_p3 ACTCCATCTCCAATGGTCCA TGCGAAGTATGTGTTCGTGT 50.0 45.0 58.0 57.9 1013-1036 bp 1.0 82.0
ndhG-ndhI_p4 ACTCCATCTCCAATGGTCCA AGGATGAGAATGGACCAGCT 50.0 50.0 58.0 58.1 1404-1427 bp 1.0 81.7
ndhG-ndhI_p5 GACATTTACGGCTCCCACAT AGGATGAGAATGGACCAGCT 50.0 50.0 58.0 58.1 1324-1347 bp 1.0 81.7